Chinese Journal of Chromatography

Chinese Journal of Chromatography

• Articles • Previous Articles     Next Articles

Determination of Three Nitroimidazole Residues in Royal Jelly by High Performance Liquid
Chromatography-Tandem Mass Spectrometry

DING Tao1, XU Jinzhong1, SHEN Chongyu1, JIANG Yuan1, CHEN Huilan1, WU Bin1, ZHAO Zengyun1, LI
Gonghai1, ZHANG Jing1, LIU Fei2

  

  1. 1.National Honey Reference Laboratory, Animal, Plant and Food Inspection Center (APFIC)
    of Jiangsu Entry-exit Inspection and Quarantine Bureau, Nanjing 210001, China;
    2.Thermo Electron (Shanghai) Corporation, Shanghai 201206, China

  • Received:2006-02-27 Revised:2006-04-30 Online:2006-07-30 Published:1986-12-25
  • Contact: DING Tao

PDF

874

Abstract:

A method for analysis of trace metronidazole (MTZ), dimetridazole (DMZ) and ronidazole
(RNZ) residues in royal jelly was developed by high performance liquid chromatography-tandem mass
spectrometry (HPLC-MS/MS). After samples were dissolved in sodium hydroxide solution to disassociate
target analytes from matrix, liquid-liquid extraction methods by ethyl acetate solvent were used.
Matrix effects were minimized and good quantitation results were obtained by using highly-selective
reaction monitoring (H-SRM) technology when deuterated dimetridazole (dimetridazole-D3) was selected
as internal standard. Limits of detection (LODs) were 1.0 μg/kg for DMZ, 0.5 μg/kg for MTZ and
RNZ (S/N＞5). Limits of quantitation (LOQs) were 2.0 μg/kg for DMZ, 1.0 μg/kg for MTZ and RNZ
（S/N＞10）.The linear ranges were 2.0-200 μg/L for all target analytes. Recoveries and relative
standard deviations (RSDs) were in the ranges of 96.6%-110.6% and 2.1%-7.4%, respectively. This
method is suitable for statutory residue testing in the National Residue Surveillance Plan in China
and meets the requirement for export.

Key words:
deuterated internal standard, nitroimidazole, royal jelly , tandem mass spectrometry (MS/MS), high performance liquid chromatography (HPLC)