Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Determination of the Molecular Mass of Modified Citrus Pectin Using High Performance Size Exclusion Chromatography

ZHANG Wenbo1,2, GAO Lin1, SHI Xiufang1, ZHANG Qilai3   

  1. 1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
    2.Tianjin Union Biotechnology Development Co., Ltd., Tianjin 300457, China; 3.Environmental Protection Bureau of Wuqing District, Tianjin 301700, China
  • Received:2007-04-05 Revised:2007-06-29 Online:2007-09-30 Published:1985-03-25

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Abstract:

Modified citrus pectin (MCP) is a polysaccharide with anti-tumor growth, anti-invasiveness and anti-metastasis activities. It is of significance to determine the molecular mass and polydispersity of polysaccharide, which could influence pharmacological activities of polysaccharide. To the best of our knowledge, no study has been reported on calculating the molecular mass of MCP by high performance size exclusion chromatography-refractive index detection (HPSEC-RID). A TSK G3000PWXL column was used at 40 ℃, and the mobile phase was composed of 50 mmol/L NH4Ac solution. Pullulan with known molecular mass was chosen as calibration standard to obtain regression equation. Relative molecular mass of MCP can be derived from the regression equation. Relative molecular masses of MCP were obtained: Mn, 21000; Mw, 43000; Mp, 46000; Mz, 66000. The polydispersity index of MCP was 2. The established method is simple, rapid and repeatable for quality control in preparing MCP and it would be also applicable to the study of composition-activity relationship of MCP in the future.

Key words: modified citrus pectin （MCP）, molecular mass , polysaccharide, high performance size exclusion chromatography （HPSEC）