Abstract:

A method for the determination of vitamin D in calcium fortified foods using solid-phase extraction-high performance liquid chromatography has been developed. The Chromabond XTR solid-phase extraction column (14500 mg, 70 mL) was used to extract and clean-up the sample. The calibration curve of vitamin D showed good linearity in the range of 0.1-100.0 μg/mL with correlation coefficient of 0.999. The limit of qualification was 0.01 μg/g and the limit of quantification was 0.03 μg/g. The average recoveries at three spiking levels were 106.2%, 99.5%, 100.1%, and the relative standard deviations were lower than 10%.

Key words: calcium fortified foods
, solid-phase extraction (SPE), vitamin D, high performance liquid chromatography (HPLC)