Abstract:

In order to perform the isolation and purification of porcine hemoglobin from porcine blood. A three-step
method, utilizing ultrafiltration, DEAE-Sepharose Fast Flow anion-exchange chromatography and Sephadex G-75 gel
exclusion chromatography in sequence, for the separation and purification of porcine hemoglobin was developed. The
obtained hemoglobin was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), high-
performance size-exclusion chromatography and reversed-phase high performance liquid chromatography. Through the
three-step purification process, the purity of the obtained hemoglobin was more than 99% with a concentration of
1.328 g/L.

Key words:
separation, anion-exchange chromatography, gel exclusion chromatography, porcine hemoglobin, purification , ultrafiltration