Abstract: An analytical method of high performance liquid chromatography (HPLC) was established for determining carbendazim residue in orange and soil. The residue was extracted with alkaline acetonitrile, cleaned-up by NH2 solid-phase extraction (SPE), separated on a Luna C18 column (250 mm×4.6 mm, 5 μm) using 10 mmol/L ammonium acetate solution (A) and acetonitrile (B) as the mobile phases with the gradient elution (0→15 min, 20%B; 15→25 min, 80%B; 25→35 min, 20%B) at a flow rate of 1.0 mL/min, and detected at 275 nm. The method showed a linear relationship between peak area and concentration at the range of 0.02~5.0 mg/L for carbendazim. The average recoveries of the method ranged from 89.2% to 102% with the relative standard deviations of 1.8%~ 9.1% at spiked level of 0.05~ 0.5 mg/kg. The limit of detection was 0.05 mg/kg in sarcocarp and soil, and that was 0.1 mg/kg in pericarp and whole orange. The method is characterized by simplicity, higher sensitivity and accuracy.

Key words: carbendazim residue, orange, soil , solid-phase extraction (SPE), high performance liquid chromatography (HPLC)