Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2010, Vol. 28 ›› Issue (11): 1107-1110.DOI: 10.3724/SP.J.1123.2010.01107

• Technical Notes • Previous Articles     Next Articles

Determination of enrofloxacin residue in chicken muscle using molecular imprinted solid phase extraction-high performance capillary electrophoresis

WANG Xueyan1, TAN Huarong2, QI Kezong3*, SHAO Li3, LI Hui3, XUE Xiuheng1, XIE Ying1   

  1. 1. College of Tea ＆ Food Science and Technology, Anhui Agricultural University, Hefei 230036, China; 2. Biotechnology Center, Anhui Agricultural University, Hefei 230036, China; 3. College of Animal Science, Anhui Agricultural University, Hefei 230036, China
  • Received:2010-07-14 Revised:2010-08-16 Online:2010-11-28 Published:2010-11-28

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Abstract: Molecular imprinted polymer targeted for enrofloxacin (ENR) was synthesized using ENR as the template molecules, α-methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking agent. With the ENR molecular imprinted polymer, a molecular imprinted solid phase extraction-high performance capillary electrophoresis (MISPE-HPCE) method for the determination of ENR in chicken muscle was developed. The results showed that this method exhibited high selectivity and sensitivity for the determination of ENR in chicken muscle. Under the optimized conditions, the limit of detection and limit of quantification were 92.02 μg/kg and 336.04 μg/kg, respectively. The recoveries of the ENR spiked in chicken muscle at different levels were in the range of 77.84%~86.52%, and the relative standard deviations (RSDs) were in the range of 2.18%~3.76%. This MISPE-HPCE method is feasible for the analysis of ENR residue in chicken muscle.

Key words: chicken muscle, enrofloxacin, high performance capillary electrophoresis (HPCE), solid phase extraction (SPE), molecular imprinted polymer (MIP)