Abstract:

An analytical method was developed for the determination of the preservative of chlorphenesin in cosmetics by high performance liquid chromatography (HPLC). A C₁₈ column (250 mm×4.6 mm, 5 μm) and a photodiode array detector were used. The mobile phase was methanol-water (55:45, v/v) with a flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm and the column temperature was 25 ℃. The limit of detection was 3 ng. A good linear relationship was obtained between the peak area and the mass concentration of chlorphenesin in the range of 1-500 mg/L and the correlation coefficient was 1.0000. The recoveries of chlorphenesin at different spiked levels were 99.0%-103% with the relative standard deviations (RSD) ≤1.2%. Interference test and sample test were also applied meanwhile and validation experiments were performed by three laboratories. The method is simple, sensitive, accurate, stable and suitable for the determination of chlorphenesin in cosmetics.

Key words: chlorphenesin, cosmetics, high performance liquid chromatography (HPLC), preservative