Chinese Journal of Chromatography ›› 2023, Vol. 41 ›› Issue (10): 937-948.DOI: 10.3724/SP.J.1123.2023.08019
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LAU Waichun#, CHEN Yali#, XIA Ling*(), XIAO Xiaohua*(), LI Gongke*()
Received:
2023-08-24
Online:
2023-10-08
Published:
2023-10-23
Supported by:
CLC Number:
LAU Waichun, CHEN Yali, XIA Ling, XIAO Xiaohua, LI Gongke. Surface-modified microchip electrophoretic separation and analysis of functional components in health care products[J]. Chinese Journal of Chromatography, 2023, 41(10): 937-948.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2023.08019
Fig. 1 Schematics of (a) the microchip electrophoresis, surface modification processes of (b) negative charge coating and (c) positive charge coating on the surface of COC COC: cycloolefin copolymer; GA: glutaraldehyde; EDC: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; NHS: N-hydroxy succinimide; EDA: ethylenediamine.
Fig. 2 (a) Survey XPS spectrum and the deconvolution analysis of (b) C 1s, (c) N 1s, and (d) O 1s XPS spectra of static coating COC surface, (e) contact angle on COC plate and (f) profiles of air-water interface in COC microchannel with negative charge coating unmodified and modified, adsorption to amino acids on COC channel surfaces with static coating (g) unmodified and (h) modified XPS: X-ray photoelectron spectroscopy.
Fig. 3 Effects of hydrophobic amino acid types on (a) the peak shape and (b) theoretical plate height (H) of arginine,effects of hydrophilic amino acid on (c) the peak shape and (d) H of arginine For b and d: n=3.
Fig. 4 Effects of operating voltage (φ) on (a) the fluidic transport rate (u) and (b) the H of Lys and GABA, measured Rs on (c) different separation distances (L) and (d) different φ of Lys and GABA (n=3)
Analyte | This method | Original with HPLC method/(mg/kg) | Relative error/% | ||||
---|---|---|---|---|---|---|---|
Original/(mg/kg) | Spiked/(mg/kg) | Found/(mg/kg) | Recovery/% | RSD/% | |||
Lys | 3.08±0.03 | 3.00 | 6.51 | 107 | 3.6 | 3.05±0.02 | 0.98 |
6.00 | 10.8 | 118 | 2.1 | ||||
12.0 | 15.7 | 104 | 1.0 | ||||
GABA | 2.53±0.15 | 3.00 | 4.69 | 84.8 | 7.2 | 2.32±0.04 | 9.1 |
6.00 | 8.25 | 96.7 | 7.2 | ||||
12.0 | 16.6 | 114 | 2.6 |
Table 1 Analysis of Lys and GABA in children’s health products (n=3)
Analyte | This method | Original with HPLC method/(mg/kg) | Relative error/% | ||||
---|---|---|---|---|---|---|---|
Original/(mg/kg) | Spiked/(mg/kg) | Found/(mg/kg) | Recovery/% | RSD/% | |||
Lys | 3.08±0.03 | 3.00 | 6.51 | 107 | 3.6 | 3.05±0.02 | 0.98 |
6.00 | 10.8 | 118 | 2.1 | ||||
12.0 | 15.7 | 104 | 1.0 | ||||
GABA | 2.53±0.15 | 3.00 | 4.69 | 84.8 | 7.2 | 2.32±0.04 | 9.1 |
6.00 | 8.25 | 96.7 | 7.2 | ||||
12.0 | 16.6 | 114 | 2.6 |
Fig. 5 Electropherograms of original and 3.00 mg/kg spiked children’s health products BGE: 0.1 mmol/L sodium tetraborate; φ: 800 V; separation distance: 2.0 cm.Peaks: 1. Lys; 2. GABA.
Fig. 6 (a) Survey XPS spectrum and the deconvolution analysis of (b) C 1s, (c) N 1s and (d) O 1s XPS spectra of static coating COC surface, (e) contact angle on COC plate and (f) profiles of air-water interface in COC microchannel with positive charge coating unmodified and modified, adsorption to aspartate and taurine on COC channel surfaces with static coating (g) unmodified and (h) modified
Fig. 7 Effect of mass fractions of HPMC and SDS on the u of (a) aspartate acid and (b) taurine, and effect of mass fractions of HPMC and SDS on the total peak width σ2 of (c) aspartate acid and (d) taurine (n=3)
Fig. 8 Effect of φ on the u of (a) aspartic acid and (b) taurine, and effect of φ on H of (c) aspartic acid and (d) taurine (n=3) For a and b, 1. dynamic only; 2. dynamic & static; 3. static only. For c and d, 1. dynamic & static; 2. static only; 3. dynamic only.
Fig. 9 (a) Effect of the coating type on the separation resolution of aspartic acid and taurine, measured Rs of aspartic acid and taurine under (b) different separation distances and (c) different φ For b and c: n=3. Peaks: 1. aspartic acid; 2. taurine.
Fig. 10 Electropherograms of original and 8.00 mg/kg spiked sport drink samples BGE: 0.1 mmol/L sodium tetraborate containing 0.005%(mass fraction) HPMC and 0.015% (mass fraction) SDS; φ: 800 V; L: 1.5 cm.Peaks: 1. aspartic acid; 2. taurine.
Analyte | This method | Original with HPLC method/(mg/kg) | Relative error/% | ||||
---|---|---|---|---|---|---|---|
Original/(mg/kg) | Spiked/(mg/kg) | Found/(mg/kg) | Recovery/% | RSD/% | |||
Asp | 9.89±0.55 | 8.00 | 18.2 | 102 | 1.0 | 9.75±0.15 | 1.4 |
16.0 | 25.3 | 97.5 | 6.4 | ||||
32.0 | 43.9 | 105 | 3.6 | ||||
Tau | 15.9±0.8 | 8.00 | 24.2 | 101 | 4.1 | 14.6±0. 1 | 9.4 |
16.0 | 37.4 | 117 | 2.6 | ||||
32.0 | 56.6 | 118 | 3.0 |
Table 2 Analysis of aspartic acid and taurine in sport drink (n=3)
Analyte | This method | Original with HPLC method/(mg/kg) | Relative error/% | ||||
---|---|---|---|---|---|---|---|
Original/(mg/kg) | Spiked/(mg/kg) | Found/(mg/kg) | Recovery/% | RSD/% | |||
Asp | 9.89±0.55 | 8.00 | 18.2 | 102 | 1.0 | 9.75±0.15 | 1.4 |
16.0 | 25.3 | 97.5 | 6.4 | ||||
32.0 | 43.9 | 105 | 3.6 | ||||
Tau | 15.9±0.8 | 8.00 | 24.2 | 101 | 4.1 | 14.6±0. 1 | 9.4 |
16.0 | 37.4 | 117 | 2.6 | ||||
32.0 | 56.6 | 118 | 3.0 |
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