Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (1): 108-112.DOI: 10.3724/SP.J.1123.2015.10035

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Enantioseparation behavior of chiral stationary phases AD, AS and OD

LI Liqun1, FAN Jun1, ZHANG Jing1, CHEN Xiaodong2, WANG Tai2, HE Jianfeng1, ZHANG Weiguang1,2   

  1. 1. School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China;
    2. Guangzhou Research & Creativity Biotechnology Co. Ltd., Guangzhou 510663, China
  • Received:2015-10-24 Online:2016-01-08 Published:2012-08-01
  • Supported by:

    Project of National Natural Science Foundation of China (21171059, 21571070); Innovation Fund for Technology Based Firms, Ministry of Science and Technology, China (13C26214404534); Science and Technology Project of Guangdong Province, China (2014A010101145, 2012B010900043); Guangzhou Science and Technology Project, China (2013J4400027, 201508020093).

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Abstract:

Over the past decades, HPLC enantioseparation with chiral stationary phases (CSPs) has been widely applied in chiral analysis and preparation of new pharmaceuticals, pesticides, food, etc. Herein, enantioseparation of 20 chiral compounds have been carried out on three polysaccharide-based CSPs (EnantioPak AD, AS and OD) with normal phases by HPLC, separately. The influences of skeletal structure and the kinds of derivative groups on separation behaviors of these CSPs have been studied in detail. As results indicated, except for compound 13, the other compounds were baseline separated on EnantioPak AD, with most of resolution over 2.0; in addition, better separation for acidic or basic compounds was achieved through adding acidic/basic additives into the mobile phase of hexane-alcohol. For four aromatic alcohols (compounds 13-16), their retention in the EnantioPak AD column showed a weakening tendency with increase of carbon number in side chain group, and the reverse trend of their resolution was observed. Furthermore, EnantioPak AD showed much better separation performance for eight compounds (13-20) than the others. In short, these results have provided some references for further investigation of separation behavior and applications of polysaccharide-based CSPs.

Key words: chiral recognition, high performance liquid chromatography (HPLC), polysaccharide-based chiral stationary phases, separation mechanism

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