Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Simultaneous determination of artificial sweeteners in beverage by ultra performance liquid chromatography

JI Chao1, SUN Yanyan2,3, LI Xiuqin2, CHU Xiaogang2, CHEN Zhengxing1*   

  1. 1.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214036, China; 2.Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100025, China; 3.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2008-05-09 Revised:2008-07-21 Online:2009-01-30 Published:1983-03-25
  • Contact: CHEN Zhengxing

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Abstract: An ultra performance liquid chromatographic (UPLC) method for the simultaneous separation and determination of four artificial sweeteners (sodium saccharin, aspartame, acesulfame and neotame) in a single injection was developed. The separation was performed on an ACQUITY UPLCTM BEH C18column with gradient program and detection at 220 nm. The good linearities between the concentrations of all analytes and peak area responses were achieved over the range from 0.5 to 20.0 mg/L. The average recoveries in samples were 80.5%-95.2% with the relative standard deviations of 0.50%-8.7%. The method has been successfully applied to the determination of the four sweeteners in drinks and powdered tabletop sweeteners.

Key words: artificial sweeteners, beverage , ultra performance liquid chromatography (UPLC)