Abstract: A rapid method for the determination of melamine in eggs was established using high performance liquid chromatography (HPLC). The melamine in a test sample was extracted with ultrasonic in 1% trichloroacetic acid for 10 min, and then purified with precipitation, centrifugation and filtration, at last tested by HPLC system. The total time of HPLC analysis for each sample was only 10 min. The linear range was from 0.05 to 20.0 mg/L as the linear correlation coefficient was 0.9999. The limit of quantification was 0.10 mg/kg (S/N＞10). The average recoveries were between 94.2% and 107% in the spiked range of 1.0~6.0 mg/kg in eggs. The relative standard deviations (RSDs) of parallel determination were between 1.53% and 2.06%(n6). This method is simple, fast, sensitive and suitable for the determination of melamine in eggs.

Key words: eggs , fast determination, melamine, high performance liquid chromatography (HPLC)