Chinese Journal of Chromatography ›› 2012, Vol. 30 ›› Issue (04): 423-427.DOI: 10.3724/SP.J.1123.2011.12055

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Preparation of molecularly imprinted polypyrrole/Fe3O4 composite material and its application in recognition of tryptophan enantiomers

CHEN Zhidong1, SHAN Xueling1, KONG Yong1,2*   

  1. 1. School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China; 2. School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China
  • Received:2011-12-19 Revised:2012-01-17 Online:2012-04-28 Published:2012-04-28

Abstract: Ferrosoferric oxide (Fe3O4) magnetic material was first synthesized, and then the in-situ chemical polymerization of pyrrole was carried out on the surface of Fe3O4 by using pyrrole and L-tryptophan (L-Trp) as the functional monomer and templates, respectively. As a result, molecularly imprinted polypyrrole/Fe3O4 composite material was obtained. This composite material was separated from the solution because of its magnetic property. Polypyrrole in the composite was overoxidized in 1 mol/L NaOH solution by applying a potential of 1.0 V, and thus L-Trp templates were de-doped from the composite. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and electrochemical methods were employed to characterize the composite. The solution containing L- or D-Trp was pumped through a porous ceramic tube packed with the composite, separately. High performance liquid chromatography (HPLC) was adopted for the detection of L- or D-Trp in the eluate, and the results indicated that the enrichment ability of the composite for L-Trp was almost 2 times of that of D-Trp. Therefore, the electro-magnetic composite material has potential applications as chromatographic stationary phase for chiral recognition.

Key words: chromatographic stationary phase, enantiomers, recognition, tryptophan, polypyrrole/Fe3O4 composite material