Reversed-phase high performance liquid chromatography separation of 2-(fluorophenyl)-5-methylbenzoxazole and 2-(chlorophenyl)-5-methylbenzoxazole positional isomers

doi:10.3724/SP.J.1123.2013.01030

Abstract

Abstract:

The analytical method of 2-(fluorophenyl)-5-methylbenzoxazole ortho-, meta-, para-positional isomers and 2-(chlorophenyl)-5-methylbenzoxazole ortho-, meta-, para-positional isomers was developed with a commercial common liquid chromatography column. The separation was performed on an Inertsil ODS-SP C₁₈ column (250 mm×4.6 mm, 5 μm) at 40℃ with a linear gradient elution by mobile phases of acetonitrile (A) (from 60% to 80% within 15 min) and water (B) at a flow rate of 1.5 mL/min. The samples were detected by a diode array detector at 310 nm. Good linearities were obtained in the range of 2-200 mg/L for the six isomers. The limits of detection (LODs) (S/N=3) of 2-(fluorophenyl)-5-methylbenzoxazole ortho-, meta-, para-isomers and 2-(chlorophenyl)-5-methylbenzoxazole ortho-, meta-, para-isomers were 0.0307, 0.0293, 0.0315, 0.0226, 0.0237, 0.0226 mg/L, respectively. The method provides not only a rapid detection method for the preparation of the isomers through hydrocarbon activation coupling reaction between the 5-methylbenzoxazole and fluorobenzene or chlorobenzene but also a reference for the separation and detection of 2-arylbenzoxazole isomers.

Key words: benzoxazole, C₁₈ column, high performance liquid chromatography (HPLC), positional isomers

CLC Number:

O658

WANG Min. Reversed-phase high performance liquid chromatography separation of 2-(fluorophenyl)-5-methylbenzoxazole and 2-(chlorophenyl)-5-methylbenzoxazole positional isomers[J]. Chinese Journal of Chromatography, 2013, 31(8): 758-762.

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