Sub-micrometer mesoporous SBA-15 silica rods as stationary-phase for capillary electrochromatography separation

doi:10.3724/SP.J.1123.2012.11016

Abstract

Abstract:

Mesoporous SBA-15 silica rods were packed into capillary as stationary phase for capillary electrochromatography (CEC) separation. The attractive properties of the sub-micrometer material are its radical size of 400 nm and highly ordered cylindrical mesopores running along the same direction. The special structure is formed by piling of rod shape particles, which provide good permeability and facilitate the packing procedure of small size CEC column. The modified silica particles were successfully applied for the reversed-phase (RP) and ion-exchange (IE) electrochromatographic separation of non-polar and polar compounds with satisfactory efficiency (140000 plates/m for benzene using 70%(v/v) acetonitrile). The van Deemter plot showed the hindrance to mass transfer because of the existence of pore structure. The lowest plate height of 7.1 mm was obtained at the linear velocity of 3.2 cm/min. The prepared ion exchange column was applied to the separation of Chinese traditional medicines Ginseng and Rhizoma Gastrodiae.

Key words: capillary electrochromatography (CEC), SBA-15 silica rods, stationary phase, sub-micrometer

CLC Number:

O658

ZHANG Lingyi, WANG Zhicong, ZHANG Weibing^*. Sub-micrometer mesoporous SBA-15 silica rods as stationary-phase for capillary electrochromatography separation[J]. Chinese Journal of Chromatography, 2013, 31(4): 335-341.

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