Abstract:

Magnetic nanoparticles of ferroferric oxide/carbon nanotubes (Fe₃O₄/CNTs) were synthesized by chemically bonding single-walled carbon nanotubes (SWCNTs) to the Fe₃O₄ nanoparticles. The pretreated carboxyl SWCNTs were modified on the surface of amino-functioned Fe₃O₄ nanoparticles through the cross-linking reaction between 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The synthesized Fe₃O₄/CNTs nanoparticles presented high super paramagnetic property and good dispersion ability, and were considered as good candidate adsorbents for dispersive solid phase microextraction. In this work, the synthesized Fe₃O₄/CNTs nanoparticles were applied to the extraction of vanillin additives, which was combined with high performance liquid chromatography (HPLC) analysis. The rapid and efficient preconcentration of vanillin and ethyl vanillin from different milk samples were successfully obtained with the detection limits of 10 μg/L and satisfactory recoveries of more than 92%. The results indicated that Fe₃O₄/CNTs magnetic nanoparticles were good pretreatment alternatives for the concentration of vanillin additives in milk products.

Key words: ferroferric oxide, high performance liquid chromatography (HPLC), magnetic nanoparticles, milk, single-walled carbon nanotubes (SWCNTs), vanillin additives