Simultaneous determination of the residues of four β2-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2015.08033

Abstract

Abstract:

An analytical method was developed to simultaneously determine the residues of four β₂-agonists in animal foods by high performance liquid chromatography/electrospray-tandem mass spectrometry (HPLC-MS/MS). Samples were extracted with 5% (mass fraction) trichloracetic acid, and then cleaned up by two solid phase extraction cartridges (HLB and ProElut PXC). Quantification of the four β₂-agonists was achieved by HPLC-MS/MS in multiple reaction monitoring (MRM) using internal standard method. The limits of detection (S/N=3) of clenbuterol, salbutamol, ractopamine and terbutaline were 0.05, 0.05, 0.05 and 0.2 μ g/kg, and the limits of quantification (S/N=10) were 0.25, 0.25, 0.1 and 0.5 μ g/kg, respectively. The average recoveries of the four β₂-agonists spiked in blank samples at the spiked levels of 2.5, 5 and 10 μ g/kg were 90.3%-120.5% with the relative standard deviation (RSD) range of 1.60%-9.33%. The method is reliable, sensitive, good recovery and repeatability. It is suitable for the determination of the residues of the four β₂-agonists in animal foods.

Key words: β₂-agonists, acidolysis extraction, animal foods, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), solid phase extraction

CLC Number:

O658

LI Juan, QIAO Qingdong, ZHUANG Jingxin, WANG Cuiping. Simultaneous determination of the residues of four β₂-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(2): 170-175.

References

[1] Andrrdr S, Jira W, Schwind K H, et al. Meat Sci, 2010, 86(1): 38
[2] Sato S, Nomura S, Kawano F, et al. J Physiol Sci, 2010, 60(2): 119
[3] Koeberl D D, Li S, Dai J, et al. Mol Genet Metab, 2012, 105(2): 221
[4] Ministry of Agriculture. No. 176 Bulletin of the Ministry of Agriculture of the People's Republic of China. (2002-02-09) [2011-04-22]. http://www.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976307.htm 农业部. 中华人民共和国农业部公告第176号. (2002-02-09) [2011-04-22]. http://www.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976307.htm
[5] Wang P L, Fan L, Su X O, et al. Chinese Journal of Analytical Chemistry, 2012, 40(3): 470 王培龙, 范理, 苏晓鸥, 等. 分析化学, 2012, 40(3): 470
[6] Huang S W, Zeng T, Ma L. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2014, 50(6): 763 黄仕稳, 曾婷, 马丽. 理化检验-化学分册, 2014, 50(6): 763
[7] Miao H, Zou J H, Fan S, et al. Chinese Journal of Chromatography, 2010, 28(6): 572 苗虹, 邹建宏, 范赛, 等. 色谱, 2010, 28(6): 572
[8] Zhang X L, Luo Y J, Jiang J, et al. Chinese Journal of Chromatography, 2015, 33(8): 838 张学亮, 罗云敬, 姜洁, 等. 色谱, 2015, 33(8): 838
[9] Xiong L, Li W H, Gao Y Q, et al. Journal of Food Safety and Quality, 2015, 6(2): 528 熊琳, 李维红, 高雅琴, 等. 食品安全质量检测学报, 2015, 6(2): 528
[10] Fan S, Miao H, Zhao Y F, et al. J Agric Food Chem, 2012, 60(8): 1898
[11] Moragues F, Igualada C. Anal Chim Acta, 2009, 637(1/2): 193
[12] Sun L, Zhang L, Zhu Y L, et al. Chinese Journal of Chromatography, 2008, 26(6): 709 孙雷, 张骊, 朱永林, 等. 色谱, 2008, 26(6): 709
[13] Liu M, Liu R, Wang L Q, et al. Journal of Instrumental Analysis, 2012, 31(3): 290 刘敏, 刘戎, 王立琦, 等. 分析测试学报, 2012, 31(3): 290
[14] Gu L, Ding L. Chemical Analysis and Meterage, 2012, 21(1): 37 顾亮, 丁磊. 化学分析计量, 2012, 21(1): 37
[15] Li Y Y, Song Y Q, Guo W P, et al. Meat Research, 2012, 26(5): 25 李莹莹, 宋永青, 郭文萍, 等. 肉类研究, 2012, 26(5): 25
[16] SANCO/12571/2013
[17] Rajski ?, Lozano A, Uclés A, et al. J Chromatogr A, 2013, 1304: 109
[18] Lozano A, Rajski ?, Uclés S, et al. Talanta, 2014, 118: 68

Simultaneous determination of the residues of four β₂-agonists in animal foods by modified high performance liquid chromatography-tandem mass spectrometry

PDF

Knowledge

Abstract

Cite this article

share this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

[1]	QIN Tongtong, GAO Li, ZHAO Wenjie. Application progress of hypercrosslinked porous organic polymers in cartridge-based solid phase extraction [J]. Chinese Journal of Chromatography, 2023, 41(7): 554-561.
[2]	XIA Baolin, WANG Shitao, YIN Jingjing, ZHANG Weiyi, YANG Na, LIU Qiang, WU Haijing. Simultaneous determination of 43 antibacterials from nine categories in water using automatic sample loading-solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(7): 591-601.
[3]	WANG Yuanyuan, LI Lulu, LÜ Jia, CHEN Yongyan, ZHANG Lan. Determination of 13 halobenzoquinone disinfection by-products in drinking water using solid phase extraction-ultra performance liquid chromatography-triple quadrupole mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(6): 482-489.
[4]	WU Shaoming, OUYANG Liqun, MENG Peng, HE Menghang, LIN Qin, CHEN Yankai, LIU Wenjing, SU Xiaoming, DAI Ming. Determination of 18 caine anesthetics in animal meat using solid phase extraction combined with ultra-performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(5): 434-442.
[5]	LU Huiyuan, WANG Lijuan, ZHANG Jiongkai, ZHANG Chizhong, LI Tianjuan, JI Ruixue, SHEN Weijian. Determination of four fatty acid ethyl esters in olive oil by solid phase extraction-gas chromatography [J]. Chinese Journal of Chromatography, 2023, 41(4): 359-365.
[6]	HAN Linxue, ZHANG Xu, HU Xiaojian, ZHANG Haijing, QIU Tian, LIN Xiao, ZHU Ying. Determination of 12 typical personal care products in human urine samples by ultra performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(4): 312-322.
[7]	WANG Jin, YE Kaixiao, TIAN Yan, LIU Ke, LIANG Liuling, LI Qingqian, HUANG Ning, WANG Xinting. Simultaneous determination of 22 antibiotics in environmental water samples by solid phase extraction-high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(3): 241-249.
[8]	ZHANG Xu, HAN Linxue, QIU Tian, HU Xiaojian, ZHU Ying, YANG Yanwei. Determination of phenoxyacetic herbicides, metabolites of organophosphorus and pyrethroid pesticides in human urine using solid phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(3): 224-232.
[9]	XUE Kunpeng, YU Lingyu, REN Xingfa, TU Bingfang, CHEN Chao, XU Ting, HE Huan, HU Shuai. Determination of 15 carbonyl compounds in soil using improved solid phase extraction-high performance liquid chromatography [J]. Chinese Journal of Chromatography, 2023, 41(3): 265-273.
[10]	LIU Zhaoyang, DONG Jun, LI Hongxia, YANG Ruiyue, SHAO Zhiyu, WANG Siming. Determination of phosphatidylethanol in whole-blood by liquid chromatography-tandem mass spectrometry based on intelligent scheduled time-zone acquisition technology and the application to population level survey [J]. Chinese Journal of Chromatography, 2023, 41(2): 131-141.
[11]	ZHAI Hongwen, MA Hongyu, CAO Meirong, ZHANG Mingxing, MA Junmei, ZHANG Yan, LI Qiang. Application progress of on-line sample preparation techniques coupled with liquid chromatography-mass spectrometry system in the detection of food hazards [J]. Chinese Journal of Chromatography, 2023, 41(12): 1062-1072.
[12]	YU Tao, CHEN Li, ZHANG Wenmin, ZHANG Lan, LU Qiaomei. Advances in synthesis methods and applications of microporous organic networks for sample preparation [J]. Chinese Journal of Chromatography, 2023, 41(12): 1052-1061.
[13]	LIU Leiqi, CHEN Jingze, FU Wusheng, TANG Cuiying. Determination of amanita peptide and tryptamine toxins in wild mushrooms by high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(11): 976-985.
[14]	ZHOU Zhiming, LI Jing, CHEN Zhanghao, WU Yingying, LU Tuliu, XIAO Shuxiong. Determination of 32 oxidative dyes by high performance liquid chromatography and confirmation by high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2022, 40(9): 797-809.
[15]	FENG Junjun, JIANG Haiyun, WANG Jing, JING Zhengyi, ZHANG Fan, TAN Tianyu, HE Feng, JIANG Lihua, LI Haiqin, CHANG Shimin, LI Tengfei. Simultaneous determination of 40 plant growth regulators, fungicides, insecticides, and antibiotics in bean sprouts by QuEChERS-high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2022, 40(9): 843-853.