Determination of three flavonoids in loquat leaves from different producing areas by high performance liquid chromatography

doi:10.3724/SP.J.1123.2016.07022

Abstract

Abstract:

A method for simultaneous determination of three flavonoids in loquat leaves was established by high performance liquid chromatography (HPLC), and the variations of contents of rutin, quercetin and kaempferol in loquat leaves from different producing areas were investigated. Powder of loquat leaves was extracted with methanol under ultrasonic extraction, then the lipid substances were recovered with hydrochloric acid before analyzed. A Diamonsil C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phases consisted of 0.4% (v/v) phosphoric acid and acetonitrile as gradient elution. The contents of rutin, quercetin and kaempferol in eight loquat leaf samples collected from seven producing areas were determined. Three flavonoids all showed good linear relationships in external standard method. The spiked recoveries of rutin, quercetin and kaempferol were 96.33%, 95.81% and 95.80%, with RSDs of 6.48%, 0.90% and 3.02%, respectively. The method can be applied in a rapid and easy way with high resolution and good repeatability. The contents of the three flavonoids in loquat leaves showed significant differences among producing areas, especially rutin. Kaempferol had been detected in all samples with a relative stable content. Kaempferol may be selected as an active ingredient for quality control of loquat leaf medicinal materials.

Key words: flavonoids, high performance liquid chromatography (HPLC), kaempferol, loquat leaves, producing areas, quercetin, rutin

CLC Number:

O658

ZHU Shita, ZHOU Qiaoling, JIN Ping, XIAO Yao. Determination of three flavonoids in loquat leaves from different producing areas by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2016, 34(10): 1011-1014.

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