Lipid analysis of Chinese cabbage leaves by ultra performance liquid chromatography-time of flight-tandem mass spectrometry

doi:10.3724/SP.J.1123.2016.09034

Abstract

Abstract:

A method for the lipid analysis of Chinese cabbage leaves was developed by ultra performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS). The lipid species, structures, fatty acid compositions and relative contents in Chinese cabbage leaves were elucidated. Acquity UPLC^TM BEH C₈ column was selected as the stationary phase. A fast and efficient chromatographic separation was accomplished within 22 min. TOF-MS scan-information dependent acquisition-product ion scan was performed in ESI positive and negative ion modes to acquire high resolution MS and MS/MS spectra in one injection. Finally, 232 lipids in Chinese cabbage leaves were identified, including 104 phospholipids, 63 glycolipids and 65 glycerides. Among them, phosphatidylcholines and monogalactosyldiacylglycerols were chiefly phospholipids and glycolipids, respectively. Meanwhile, glycerides mainly contained diacylglycerols and triacylglycerols. The results demonstrated that the method for lipid analysis has high sensitivity, good accuracy and high throughput. It provides a reliable analysis platform for lipid metabolism in plants and lay a foundation for further study on biological functions of lipids.

Key words: Chinese cabbage, leaves, lipid, ultra performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS)

CLC Number:

O658

ZHENG Shuning, ZHANG Yanguo, LÜ Jun, XU Donghui. Lipid analysis of Chinese cabbage leaves by ultra performance liquid chromatography-time of flight-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(2): 169-177.

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