Rapid identification of flavonoid compounds in Pericarpium Citri Reticulatae by online extraction-high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry

doi:10.3724/SP.J.1123.2017.11031

Abstract

Abstract:

A simple method was developed for the rapid extraction, separation and identification by online extraction (OLE) coupled with high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry (HPLC-DAD-QTOF-MS). For OLE strategy, a guard column filled with Pericarpium Citri Reticulatae powder (2.0 mg) was connected to manual injection valve by replacing the sample loop. Components were directly extracted and transferred to HPLC-DAD-QTOF-MS system by mobile phases without any extra operation. In comparison with offline heat-reflux extraction, OLE strategy presented higher extraction efficiency. A total of twenty-four flavonoid compounds were detected from Pericarpium Citri Reticulatae according to their retention times, UV spectra, accurate molecular masses, and fragment ions in MS/MS spectra. Neoeriocitrin, limocitrin-3-O-(3-hydroxy-3-methylglutarate)-glucoside and its isomer were discovered for the first time. It is concluded that the developed method offers new perspectives for the rapid separation and identification of natural products.

Key words: Pericarpium Citri Reticulatae, flavonoid compounds, high performance liquid chromatography (HPLC), identification, online extraction (OLE), quadrupole time-of-flight mass spectrometry (QTOF-MS)

CLC Number:

O658

TONG Chaoying, PENG Mijun, SHI Shuyun. Rapid identification of flavonoid compounds in Pericarpium Citri Reticulatae by online extraction-high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(3): 278-284.

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