Simultaneous determination of 21 illegally added compounds in health foods by ultra high performance liquid chromatography-tandem mass spectrometry with solid phase extraction

doi:10.3724/SP.J.1123.2019.01016

Abstract

Abstract: A rapid method for the simultaneous determination of 21 illegally added compounds in health foods was developed based on solid phase extraction (SPE) combined with ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). After ultrasonic extraction with acetonitrile, the samples were purified on an HLB SPE column, separated on a Waters BEH C18 column (100 mm×2.1 mm, 1.7 μm) using 10 mmol/L ammonium acetate aqueous solution and methanol as the mobile phases. The compounds were detected by an electrospray ionization (ESI) source in positive ion mode with multi-reaction monitoring (MRM). The 21 illegal additives showed good linear relationships with correlation coefficients no less than 0.995. The limits of detection (LODs) were 3-160 μg/kg for different matrixes. The recoveries ranged from 61.8% to 109.3%, and the relative standard deviations ranged from 1.6% to 14.7%. The method can be used for the simultaneous determination of the illegally added drugs in the losing weight, lipid-lowering, anti-diabetic and anti-hypertension health foods.

Key words: health foods, illegally additives, solid phase extraction (SPE), ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)

CLC Number:

O658

XU Dunming, LAI Guoyin, CHEN Yan, LUO Chao, YI Xionghai, DENG Xiaojun, FENG Feng, ZHANG Feng. Simultaneous determination of 21 illegally added compounds in health foods by ultra high performance liquid chromatography-tandem mass spectrometry with solid phase extraction[J]. Chinese Journal of Chromatography, 2019, 37(7): 778-785.

References

[1] Tu F L, Wang Y F. China Licensed Pharmacist, 2016, 13(4):18 涂凤莲, 汪元符. 中国执业药师, 2016, 13(4):18
[2] Sui H S, Wang L J, Xie F. China Pharmaceuticals, 2015, 24(22):110 隋海山, 王立娟, 谢菲. 中国药业, 2015, 24(22):110
[3] Cheng Y, Tan S Z, Jiang D J. Journal of Food Safety and Quality, 2018, 9(15):4078 程燕, 谭顺中, 姜登军. 食品安全质量检测学报, 2018, 9(15):4078
[4] He R S, Huang Y T, Lei Y, et al. Journal of Instrumental Analysis, 2016, 35(11):1486 何睿偲, 黄艳婷, 雷毅, 等. 分析测试学报, 2016, 35(11):1486
[5] Li X L, Chen J, Yang C F, et al. Food Science, 2018, 39(18):320 李晓蕾, 陈军, 杨朝芬, 等. 食品科学, 2018, 39(18):320
[6] Ni Z, Zhang C S. Central South Pharmacy, 2018, 16(9):1295 倪赞, 张崇生. 中南药学, 2018, 16(9):1295
[7] Zhang Q Y, Luo H T, Huang X L, et al. Journal of Instrumental Analysis, 2018, 37(9):989 张秋炎, 罗辉泰, 黄晓兰, 等. 分析测试学报, 2018, 37(9):989
[8] Dong Y, Jiang X, Liu J, et al. Journal of Food Safety and Quality, 2018, 9(15):4055 董宇, 姜鑫, 刘静, 等. 食品安全质量检测学报, 2018, 9(15):4055
[9] Sun J, Hu Q, Zhang S, et al. Journal of Instrumental Analysis, 2018, 37(4):419 孙健, 胡青, 张甦, 等. 分析测试学报, 2018, 37(4):419
[10] Zheng W Y, Li Z, Chen M, et al. Journal of Shenyang Pharmaceutical University, 2018, 35(1):58 郑文燕, 李哲, 陈梅, 等. 沈阳药科大学学报, 2018, 35(1):58
[11] Hu Q, Sun J, Yu H, et al. Journal of Food Safety and Quality, 2018, 9(1):93 胡青, 孙健, 于泓, 等. 食品安全质量检测学报, 2018, 9(1):93
[12] Zheng J, Xi C X, Cao S R, et al. Chinese Journal of Chromatography, 2017, 35(12):1257 郑佳, 郗存显, 曹淑瑞, 等. 色谱, 2017, 35(12):1257

[13] Xu L, Wu Y Y, Shou L J, et al. Science and Technology of Food Industry, 2017, 38(13):248 许立, 吴鸳鸯, 寿林均, 等. 食品工业科技, 2017, 38(13):248
[14] Xian R Q, Shi F, Mou W W, et al. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2017, 53(1):44 咸瑞卿, 石峰, 牟卫伟, 等. 理化检验-化学分册, 2017, 53(1):44
[15] Zhu W X, Liu Y F, Sun Z L, et al. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2016, 52(11):1286 祝伟霞, 刘亚风, 孙转莲, 等. 理化检验-化学分册, 2016, 52(11):1286
[16] Zhan S Y, Zhu Q F, Xu H X, et al. Chinese Traditional and Herbal Drugs, 2016, 47(17):3023 詹淑玉, 朱琦峰, 徐宏祥, 等. 中草药, 2016, 47(17):3023
[17] Chen L, Wen J X, Qi C Y, et al. Journal of Instrumental Analysis, 2016, 35(8):937 陈林, 温家欣, 齐春艳, 等. 分析测试学报, 2016, 35(8):937
[18] Gong X, Lu L, Feng Y L, et al. Chinese Journal of Pharmaceutical Analysis, 2016, 36(5):918 宫旭, 芦丽, 冯有龙, 等. 药物分析杂志, 2016, 36(5):918
[19] Hu Q, Sun J, Feng R, et al. Chinese Journal of Chromatography, 2017, 35(6):594 胡青, 孙健, 冯睿, 等. 色谱, 2017, 35(6):594

[20] Yang Z, Peng Y, Jin M N, et al. Chinese Journal of Chromatography, 2018, 36(9):889 杨直, 彭彦, 金朦娜, 等. 色谱, 2018, 36(9):889

[21] Wen J X, Wu F D, Cao Y J, et al. Chinese Journal of Chromatography, 2018, 36(12):1284 胡青, 孙健, 冯睿, 等. 色谱, 2018, 36(12):1284

[22] Yu H, Hu Q, Sun J, et al. Chinese Journal of Chromatography, 2018, 36(10):1005 于泓, 胡青, 孙健, 等. 色谱, 2018, 36(10):1005

[23] Li X, Tan L L, Liu J C, et al. Spectroscopy and Spectral Analysis, 2018, 38(3):830 李想, 谭丽丽, 刘吉成, 等. 光谱学与光谱分析, 2018, 38(3):830
[24] Wu G P, Lei Y P, Li Y B, et al. China Pharmaceuticals, 2010, 19(14):42 吴公平, 雷玉萍, 黎银波, 等. 中国药业, 2010, 19(14):42