Preparation and characterization of polyaniline/graphene-coated anion-exchange chromatographic stationary phase

doi:10.3724/SP.J.1123.2019.08034

Abstract

Abstract:

A polyaniline/graphene (PANI/G) composite was selected as the coating material to prepare a coated anion-exchange chromatographic stationary phase. First, aniline and graphene were used to prepare the PANI/G composite, which was coated onto the surface of poly(styrene-divinylbenzene) (PS-DVB) microspheres by physical absorption. Then, a series of anion-exchange stationary phases with different exchange capacities were obtained by quaternization using the nitrogen atom of polyaniline as the reactive site. The stationary phase was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and elemental analysis (EA). The results revealed that PANI/G was successfully coated onto the surface of PS-DVB and quaternized. The efficiency of the self-fabricated chromatographic column was evaluated by separating conventional anions and organic acids. The PANI/G-coated PS-DVB anion-exchange chromatographic column that was quaternized eight times showed good separation performance for conventional anions and organic acids.

Key words: polyaniline/graphene (PANI/G), poly(styrene-divinylbenzene) (PS-DVB), quaternization, anion-exchange, stationary phase

CLC Number:

O658

LIU Junwei, SUN Yan, YU Wenhao, WANG Chaowei, LI Zhenxing, SUN Yu’an, ZHANG Shusheng, ZHU Yan. Preparation and characterization of polyaniline/graphene-coated anion-exchange chromatographic stationary phase[J]. Chinese Journal of Chromatography, 2020, 38(4): 458-463.

Figures/Tables 4

Fig. 1 Scanning electron micrographs of (a, b) PS-DVB microspheres and (c) PANI/G coated PS-DVB microspheres PS-DVB: poly(styrene-divinylbenzene); PANI/G: polyaniline/graphene.

Fig. 2 FT-IR spectra of (a) PS-DVB, (b) PANI/G coated PS-DVB, and PANI/G coated PS-DVB quaternized (c) twice, (d) quartic, (e) sextic, and (f) eight times

Fig. 3 Separation of six conventional anions by PANI/G coated stationary phase quaternized eight-times Mobile phase: 4.5 mmol/L Na2CO3+0.8mmol/L NaHCO3; flow rate: 1.0 mL/min; injection volume: 25 μL. Peak Nos.: 1. F- (0.2 mmol/L); 2. Cl- (0.2 mmol/L); 3. N O 2 - (0.2 mmol/L); 4. Br- (0.2 mmol/L); 5. N O 3 - (0.2 mmol/L); 6. S O 4 2 - (0.2 mmol/L).

Fig. 4 Separation of four organic acids by PANI/G coated stationary phase quaternized eight times Mobile phase: 4.5 mmol/L Na2CO3+0.8mmol/L NaHCO3; flow rate: 1.0 mL/min; injection volume: 25 μL. Peak Nos.: 1. acetic acid (2.0 mmol/L); 2. formic acid (2.0 mmol/L); 3. propionic acid (5.0 mmol/L); 4. butyric acid (1.0 mmol/L).

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