Abstract:

Sinapine, an important natural antioxidant, is often obtained from cruciferous plants. Many scientists are interested in it because of its great potential in the field of antiageing drugs. Thus, the distribution of sinapine in 4 commonly used crude drugs from cruciferous plants (Semen Sinapis Albae, Semen Brassicae Junceae, Semen Raphani and Semen Lepidii) was investigated. The determination was performed by reversed-phase high performance liquid chromatography (RP-HPLC) using an Alltima Phenyl column (250 mm×4.6 mm i.d., 5 μm). A gradient elution with mobile phase consisting of (A) 3% acetic acid in water and (B) acetonitrile was used. The detection wavelength was set at 326 nm and the column temperature was kept at 25 ℃. It is an accurate, rapid and reproducible method. The results are informative for the research of a quality control tool for these natural drugs.

Key words: antioxidant, Semen Brassicae Junceae, Semen Lepidii , Semen Raphani, Semen Sinapis Albae, sinapine, high performance liquid chromatography (HPLC)