Abstract:

A method of reversed-phase high performance liquid chromatography coupled with fluorescence detection was developed for the separation and determination of papaverine in seeds of Papaver somniferum L. and soup of chafing dish. The liquid chromatographic experiment was carried out under the following conditions: the detector was a fluorescence with excitation wavelength at 285 nm and emission wavelength at 355 nm. An RP-C18 column (250 mm×4.6 mm i.d., 5 μm) was used. The mobile phase was a mixture of methanol and 0.02 mol/L ammonium acetate (70∶30, v/v). The flow rate was 0.8 mL/min. A linear range was obtained from 0.1 ng to 0.1 μg with a good correlation. The lowest detection limit was 0.02 ng. The average recovery of seeds for Papaver somniferum L. was 99.0%-100.8%. The method is rapid, accurate, sensitive and suitable for the separation and determination of papaverine in foods.

Key words: fluorescence detection, papaverine, seed of Papaver somniferum L, soup of chafing dish , high performance liquid chromatography