Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Comparison in Hydrophobic Selectivity for Two Types of Reversed-Phase High Performance Liquid
Chromatography Packing Material

GE Jin1,2, ZHANG Hongli1, LI Xiujuan1,2, SHAO Shijun1, LI Yongmin1, CHEN Liren1

  

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China;
    2.Graduated School of the Chinese Academy of Sciences, Beijing 100039, China

  • Received:2005-08-02 Revised:2005-11-15 Online:2006-07-30 Published:1986-12-25
  • Contact: Chen Liren

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Abstract:

Two types of novel reversed-phase packing materials were made by adding TiO2/SiO2 (or
ZrO2/SiO2) and octadecyltrichlorosilane into toluene with stirring and refluxing for 36 h. TiO2/SiO2
and ZrO2/SiO2 particles were prepared by a layer-by-layer self-assemble technique and consist of
micrometer-sized silica spheres as cores and nanometer-sized zirconia or titania as surface coating.
The carbon loading of C18-bonded TiO2/SiO2 was 11.51% and that of C18-bonded ZrO2/SiO2 was 9.62%.
The hydrophobic selectivity and sensitivity were studied respectively and compared in details; the
results showed that the packing supports both acted as true reversed chromatographic stationary
phase with similar hydrophobic selectivity. The chromatographic behaviors of the two types of novel
reversed-phase packing material were evaluated with nine aromatic compounds. The chromatographic
studies showed that either of the packing materials would be a good choice to separate aromatic
compounds, however, the selectivity of C18-bonded TiO2/SiO2 was a little better than that of
C18-bonded ZrO2/SiO2.

Key words: octadecyl-bonded stationary phase, silica , titania
(TiO2), zirconia (ZrO2), high performance liquid chromatography (HPLC)