Chinese Journal of Chromatography

Chinese Journal of Chromatography

• Articles • Previous Articles     Next Articles

Determination of Egg-Yolk Phosphatidylcholine by Normal-Phase High Performance Liquid Chromatography
with Evaporative Light Scattering Detection

GONG Yan1, WANG Qiao’e2, YANG Yi1, DING Mingyu2

  

  1. 1.Department of Science, Beijing University of Chemical Technology, Beijing 100029, China;
    2.Key Lab of Bioorganic Phosphorous Chemistry & Chemical Biology, Ministry of Education,
    Department of Chemistry, Tsinghua University, Beijing 100084, China

  • Received:2005-09-27 Revised:2006-02-09 Online:2006-07-30 Published:1986-12-25
  • Contact: DING Mingyu

PDF

1026

Abstract:

A method for the determination of egg-yolk phosphatidylcholine by normal-phase high
performance liquid chromatography (NP-HPLC) with evaporative light scattering detection (ELSD) was
developed. Yolk phosphatidylcholine was successfully separated on a Nova-Pak Silica 60A column (3.9
mm i.d.×150 mm, 4 μm), using hexane-isopropanol-water containing 3% acetic acid (35∶65∶8, v/v/v)
as mobile phase at a flow rate of 1.0 mL/min. The drift tube temperature and air carrier gas
pressure of the ELSD were set at 50 ℃ and 350 kPa, respectively. The linear detection range was
0.16-1.61 g/L (r2=0.9979) and the limit of detection (LOD) was 0.64 μg with excellent relative
standard deviation (RSD) of 3.2% (n=5). The recovery was in the range of 98.2%-128.2%. The results
indicated that this method is accurate, rapid, simple and reproducible, and thus suitable for the
analysis of egg-yolk phosphatidylcholine in real samples.

Key words: egg-yolk phosphatidylcholine (EYPC) , evaporative light scattering detection
(ELSD), phosphatidylcholine (PC), high performance liquid chromatography (HPLC)