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Simultaneous determination of six cucurbitane triterpene glycosides in Siraitia grosvenorii fruits using high performance liquid chromatography
LU Fenglai, LIU Jinlei, HUANG Yonglin, LI Dianpeng
2008, 26 (4):
504-508.
Siraitia grosvenorii, a traditional Chinese fruit, belongs to the family Cucurbitaceae and has been used as a pulmonary demulcent and emollient for the treatment of dry cough, sore throat, dire thirst, and constipation in folk medicine. A high performance liquid chromatographic method was developed for simultaneous determining the contents of mogrosideⅤ, mogrosideⅣA, mogrosideⅢ, 11-oxomogrosideⅢ, mogrosideⅡE and 11-oxomogrosideⅡE in Siraitia grosvenorii fruits. The chromatographic analysis was carried out on a ZORBAX SB-C18 column (150 mm×4.6 mm, 5 μm). The mobile phase was water (A) and acetonitrile (B) with gradient elution (0－3 min, 20%B-30%B; 3-8 min, 30%B-35%B; 8-9 min, 35%B). The flow rate was maintained at 0.8 mL/min. The detection wavelength was set at 203 nm and the column temperature was controlled at 30 ℃. The sample injection volume was 10 μL. The calibration curves were linear over the ranges of 0.04-1.0 mg/mL, 0.011-0.68 mg/mL, 0.010-0.80 mg/mL, 0.0097-0.58 mg/mL, 0.025-1.0 mg/mL and 0.013-0.76 mg/mL (r>0.9991) for the above cucurbitane triterpene glycosides, respectively. The average recoveries were 99.65% for mogrosideⅤ, 101.6% for mogroside ⅣA, 97.05% for mogroside Ⅲ, 103.1% for 11-oxomogrosideⅢ, 99.25% for mogrosideⅡE, and 103.0% for 11-oxomogrosideⅡE, with the relative standard deviations of 0.83%, 3.1%, 1.9%, 3.3%, 0.59% and 2.0%, respectively. This simple, rapid and accurate method is suitable for quality control and determination of raw materials and products of Siraitia grosvenorii fruits.
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