Abstract: method was established for the simultaneous determination of nine heterocyclic aromatic amines (HAAs) in mutton products by solid phase extraction-high performance liquid chromatography (SPE-HPLC). As a result, the sample was prepared by ultrasound in 2 mol/L NaOH, and dichloromethane was selected as the extraction solvent. The extract was purified and concentrated with an MCX SPE column. The chromatographic separation was achieved on a reverse-phase C18 column by gradient elution using 0.01 mol/L phosphoric acid (adjusted to pH 3.6 by triethylamine) and acetonitrile, detected with a diode array detector (DAD) at 228 nm for 2-amino-9H-pyrido［2,3-b］indole (AaC) and 2-amino-3-methyl-9H-pyrido［2,3-b］indole (MeAaC); 253 nm for 2-amino-3-methylimidazo［4,5-f］quinoline (IQ), 1-methyl-9H-pyrido［3,4-b］indole (Harman), 9H-pyrido［3,4-b］indole (Norharman); 263 nm for 2-amino-3,8-dimethylimidazo［4,5-f］quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo［4,5-f］quinoxaline (4,8-DiMeIQx), 3-amino-1-methyl-5H-pyrido［4,3-b］indole (Trp-p-2); 321 nm for 2-amino-1-methyl-6-phenylimidazo［4,5-b］pyridine (PhIP). The results showed that the nine HAAs can be separated efficiently. The recoveries (n=6) of the nine HAAs spiked in meat were between 50.27% and 94.77% with the relative standard deviations of 0.08%~4.42%. The limits of detection of this method were 1.6~41.0 μg/L for the nine HAAs. The method is simple, accurate, rapid and repeatable. It can be used for the simultaneous determination of the nine HAAs in mutton products.

Key words: heterocyclic aromatic amines, high performance liquid chromatography (HPLC), mutton products, solid phase extraction (SPE)