Chinese Journal of Chromatography ›› 2013, Vol. 31 ›› Issue (6): 572-576.DOI: 10.3724/SP.J.1123.2012.12013

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Determination of atracurium and laudanosine in dog plasma during cardiopulmonary bypass by high performance liquid chromatography with fluorometric detection

YANG Yingying1,4, CHEN Mei2, KUANG Yushan3, YE Liming3, ZHANG Wensheng4   

  1. 1. Shanxi Institute of Medicine and Life Sciences, Taiyuan 030006, China;
    2. Ouyi Pharma Co., Ltd., Shijiazhuang Pharmaceutical Group, Shijiazhuang 050051, China;
    3. West China School of Pharmacy, Sichuan University, Chengdu 610041, China;
    4. Laboratory of Anesthesia and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2012-12-05 Revised:2013-01-18 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2012.12013

Abstract:

A high performance liquid chromatographic method coupled with fluorometric detection has been developed for the determination of atracurium and its major metabolite laudanosine in dog plasma. The separation of atracurium and laudanosine was performed on an Agilent Eclipse Plus C18 column, and the mobile phase consisted of 0.03 mol/L dipotassium hydrogen phosphate and acetonitrile (72:28, v/v) at a flow rate of 1.0 mL/min. Verapamil was used as the internal standard. The sample was extracted by dichloromethane, concentrated and dissolved in the mobile phase. The detection is performed at 240 nm for excitation and 320 nm for emission. The results showed that the linear concentration ranges of the calibration curve were 25-5000 μ g/L for atracurium (r=0.9990), and 25-6000 μ g/L for laudanosine (r=0.9984). The recoveries were 92.1%-109.5%. The limits of detection were 3 μ g/L for atracurium and 1 μ g/L for laudanosine. The RSDs of intra-day and inter-day were less than 10%. The stability tests under various conditions have been performed. The method is specific, sensitive and accurate in the determination of atracurium and laudanosine, and also can be used for the pharmacokinetic investigations of atracurium and laudanosine in plasma.

Key words: atracurium, dog plasma, fluorometric detection (FLD), high performance liquid chromatography (HPLC), laudanosine, plasma concentration

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