Chinese Journal of Chromatography ›› 2013, Vol. 31 ›› Issue (6): 510-513.DOI: 10.3724/SP.J.1123.2013.01003

• Quantitative Proteomics • Previous Articles     Next Articles

Detection of the major milk allergenα-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode

FENG Xiaoyan1, ZHANG Jin2, LV Meiling2, GAO Mingxia1, ZHANG Xiangmin1   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Agilent Technologies (China) Co., Ltd., Beijing 100102, China
  • Received:2013-01-05 Revised:2013-02-02 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2013.01003

Abstract:

A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein α-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0.5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of α-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.

Key words: α-casein, food, liquid chromatography-mass spectrometry(LC-MS), milk allergen, multiple reaction monitoring (MRM)

CLC Number: