Chinese Journal of Chromatography ›› 2013, Vol. 31 ›› Issue (6): 503-509.DOI: 10.3724/SP.J.1123.2013.03056

• Quantitative Proteomics • Previous Articles     Next Articles

Recent progress in multi-stage mass spectrometry-based quantitative proteomics

ZHANG Ying, YANG Pengyuan, LU Haojie   

  1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
  • Received:2013-03-29 Revised:2013-04-16 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2013.03056

Abstract:

With the development of proteome research, quantitative proteomics is becoming a very active area of proteomics research. The development of quantitative proteomics requires the advancement of a number of mass spectrometry (MS)-based quantitative techniques. According to the mass spectrometric stage at which the peptides are quantified, MS-based quantitative methods can be divided into two categories: MS1-based quantitation and MSn-based quantitation. In this review, the progress of MSn-based quantitation approaches is summarized and the advantages and disadvantages are compared. The trend of MSn-based quantitation is also prospected.

Key words: mass spectrometry (MS), proteomics, quantitative, review

CLC Number: