Progress in preparation of open tubular columns in capillary liquid chromatography and their applications in proteomics studies

doi:10.3724/SP.J.1123.2015.11033

Abstract

Abstract:

With the further development of life sciences in recent years, the demand for analysis of very small amounts of sample has increased, and more attention has been paid for the miniaturized liquid chromatography system. Longer open tubular capillary columns were selected to increase column efficiency due to their low column back pressure, and they were often used to separate complex biological samples in high resolution. Therefore, they emerged as the new area of liquid chromatography columns. The preparation methods and applications in proteomics of open tubular columns in capillary liquid chromatography (capLC) are reviewed.

Key words: capillary liquid chromatography (capLC), open tubular capillary columns, porous layer, proteomics, review

CLC Number:

O658

ZHOU Shanshan, ZHAI Rui, JIAO Fenglong, HAO Feiran, LV Yayao, QIAN Xiaohong, ZHANG Yangjun. Progress in preparation of open tubular columns in capillary liquid chromatography and their applications in proteomics studies[J]. Chinese Journal of Chromatography, 2016, 34(3): 226-231.

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