Recent advances on exosomes isolation methods and proteomic analysis

doi:10.3724/SP.J.1123.2016.08036

Abstract

Abstract:

Exosomes are membrane-bound vesicles secreted by cells with the diameter of 30-100 nm and the density of 1.10-1.18 kg/L. Exosomes exist widely in almost all kinds of biofluids and contain a series of biologically important molecules, such as lipids, proteins, mRNAs, miRNAs and ncRNAs. Emerging evidence shows that exosomes could establish an intercellular information transfer system, thereby affecting the physiological status of cells and involving in various pathological processes. Herein, the recent advances on exosome isolation and purification methods are reviewed. In the meantime, the proteomic analysis of exosomes is briefly introduced. Finally, the future of exosome proteomic research is discussed.

Key words: exosomes, isolation and purification, proteomic, quantitative analysis, review

CLC Number:

O658

WENG Yejing, SUI Zhigang, ZHANG Lihua, ZHANG Yukui. Recent advances on exosomes isolation methods and proteomic analysis[J]. Chinese Journal of Chromatography, 2016, 34(12): 1131-1136.

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