Chinese Journal of Chromatography ›› 2017, Vol. 35 ›› Issue (4): 439-444.DOI: 10.3724/SP.J.1123.2016.09037

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Simultaneous separation of seven water-soluble vitamins with microemulsion liquid chromatography

WU Hongxing, ZHENG Aini, SU Caoyin, YU Nuojun, LI Ning   

  1. Guangdong Pharmaceutical University, Guangzhou 510006, China
  • Received:2016-09-19 Online:2017-04-08 Published:2013-07-30
  • Supported by:

    National Natural Science Foundation of China (No. 81173525).

Abstract:

A novel method was developed for the separation of seven water-soluble vitamins (VB1, VB2, VB6, VB12, VC, folic acid and nicotinamide) with microemulsion liquid chromatography. The impacts of following factors on the separation selectivity were investigated:the type of surfactant, the type of oil phase, the type of co-surfactant, the pH of mobile phase and the column temperature. The optimal conditions for the separation of the seven water-soluble vitamins were as follows:Agilent TC C18 (250 mm×4.6 mm, 5 μm) was used at 30℃; the microemulsion mobile phase was sodium dodecyl sulfate (SDS)/polyoxyethylene lauryl ether (Brij35)/n-butanol/ethyl acetate/water (2:60:66:80:864, mass ratio). Baseline separation of the seven water-soluble vitamins was achieved within 20 min with a flow rate of 0.5 mL/min and the detection wavelength of 254 nm. The calibration curve showed a good linearity with the correlation coefficients above 0.9991 in the linear range from 4 to 36 mg/L. The mean recoveries of VB1, VB2, VB6, VC and nicotinamide were 93.9%-102.9% at different spiked levels. The optimized and validated method can be used for simultaneous determination of water-soluble vitamins in food and pharmaceuticals.

Key words: microemulsion liquid chromatography (MELC), separation selectivity, water-soluble vitamins

CLC Number: