Determination of 20 perfluorinated alkyl substances in honey by high performance liquid chromatography- tandem mass spectrometry

doi:10.3724/SP.J.1123.2016.12016

Abstract

Abstract:

A sensitive method for simultaneous determination of 20 perfluorinated alkyl substances (PFASs) in honey was developed by QuEChERS-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted by acetonitrile with 1.5% (v/v) formic acid, and purified by C₁₈ and N-propylethylenediamine (PSA) adsorbents. The separation of the 20 PFASs was performed on an Atlantis T3 C₁₈ column using gradient elution with methanol containing 5 mmol/L ammonium acetate and 5 mmol/L ammonium acetate as mobile phases within 16 min. The PFASs were analyzed under the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI^-). Under the optimal conditions, the isotope internal standard method was used to quantify the contents of the 20 PFASs. The calibration curves were acquired in the concentration range of 0.2-10 μg/L with correlation coefficients (r) greater than 0.99. The limits of detection (LODs) and limits of quantitation (LOQs) ranged from 0.04 to 0.10 μg/kg and 0.10 to 0.20 μg/kg, respectively. The recoveries of the 20 PFASs spiked in blank honey samples were between 72.6% and 113.0% with relative standard derivations (RSDs) of 0.4%-15.9% (n=6). The developed method is rapid, efficient and accurate, and suitable for the simultaneous analysis of the 20 PFASs in honey samples.

Key words: high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), honey, perfluorinated alkyl substances (PFASs), QuEChERS

CLC Number:

O658

LI Shuai, CHEN Hui, JIN Linghe, PENG Tao, FAN Chunlin, WANG Minglin. Determination of 20 perfluorinated alkyl substances in honey by high performance liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(5): 495-501.

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