Analysis of traditional Chinese medicine components by high performance liquid chromatography with diode array detection based on double qualitative principles

doi:10.3724/SP.J.1123.2018.02012

Abstract

Abstract:

The double qualitative principle is a new composite qualitative method based on retention time and the characteristic peaks of the absorption spectra. Using a self-designed and assembled diode array detector (DAD), a high performance liquid chromatography (HPLC) system was constructed. The illegal additive auramine O in six kinds of herbal slices and the active ingredient schisandrin in Jujube kernel Tianma capsules were separated and qualitative analyzed using the HPLC-DAD system. The results showed that there were similar peaks in the chromatograms of pollen typhae and Jujube kernel Tianma capsules when comparing the target analytes. However, the probabilities of the targets were excluded by comparing the absorption spectra. The application results indicated that, based on the double qualitative principle of retention time/absorption spectrum, the interference of impurities in the samples could be well eliminated and the false positives could be avoided. This provides a reference method for the study of traditional Chinese medicine components.

Key words: auramine O, diode array detector (DAD), false positive, high performance liquid chromatography (HPLC), schisandrin

CLC Number:

O658

TANG Tao, YANG Sandong, ZHAO Haiqing, TAN Yimeng, FENG Jiao, XIA Mingzhu, LI Tong. Analysis of traditional Chinese medicine components by high performance liquid chromatography with diode array detection based on double qualitative principles[J]. Chinese Journal of Chromatography, 2018, 36(8): 766-771.

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