Abstract:

Atrazine is a triazine pesticide that interferes with normal physiological activities, induces oxidative stress, and is significantly toxic to plants. Therefore, the ability to quantitatively detect atrazine and study the mechanisms responsible for its toxicity are crucial for ensuring food safety and maintaining plant growth. Herein, a method was developed by QuEChERS combined with fluorescence derivatization to rapidly, sensitively, and quantitatively detect atrazine in real samples. This approach offers excellent selectivity and high sensitivity, and is simple to operate. In addition, we also investigated the effect of atrazine on catalase activity through enzyme-activity assays and molecular-docking studies to explore the mechanism responsible for the toxicity of atrazine on the molecular level. This experiment involves knowledge related to sample pretreatment, spectroscopy, and molecular docking, while the instruments used are widely available in colleges and universities and are very suitable for teaching open experiments. This experiment aims to provide students with a comprehensive understanding of experimental operations integrated with theory, as well as applied research aimed at helping students understand the use of separation materials, the selection and application of fluorescent derivatization reagents, the principles of analytical instruments, the use of enzymes, and the principles of molecular docking. This experiment is expected to enhance students’ awareness of people’s livelihoods, analytical chemistry concepts, and operational standards, which will guide the development of their scientific research thinking and improve their abilities to solve real-world problems.

Key words: open experiment, QuEChERS, fluorescence detection, atrazine, catalase, molecular docking