Abstract: A high performance liquid chromatographic (HPLC) method was developed for the determination of 22 components in hair dyes. The target analytes were separated on an amide bonded C16 silica column (250 mm×4.6 mm, 5 μm), employing acetonitrile-0.025 mol/L phosphate buffer (pH 6.0) containing 0.1% 1-heptanesulfonic acid sodium salt as the mobile phase and detected by a photodiode array detector (DAD) with the detection wavelengths of 260 nm and 280 nm. The flow rate was 1.0 mL/min and the column temperature was 25 ℃. The linear range was from 10 mg/L to 500 mg/L with good relationship. The relative standard deviations were less than 10%(except toluene-2,5-diamine sulfate, 2-methylresorcinol, N,N-diethyltoluene-2,5-diamine HCl at low concentrations) and the recoveries were from 77.6% to 122.8%. The method is simple, rapid and accurate, and is suitable for the analysis of various hair dyes.

Key words: dyes, hair dyes , high performance liquid chromatography (HPLC)