Abstract:

The chromatographic fingerprint of Chinese traditional medicine, Yinhuang Oral Liquid
together with its original materials Radix Scutellariae and Flos lonicera was established by high
performance liquid chromatography (HPLC). At the same time, baicalin and chlorogenic acid in the
Yinhuang Oral Liquid were also quantitatively determined. The analysis was performed on a
Lichrospher C18 column (250 mm×4.6 mm i.d., 5 μm) and with gradient elution. The optimum
conditions were explored. The mobile phase A was methanol and the mobile phase B was 0.1%H3PO4
aqueous solution. The wavelength of 254 nm was selected for detection. Finally, the data of
fingerprints of 6 samples of Yinhuang Oral Liquid were processed with two different mathematic
methods. This proposed method demonstrated good stability for the 6 samples of Yinhuang Oral Liquid.
In the comparison of the common peaks in chromatogram of Yinhuang Oral Liquid with those of crude
herbs, most of them could be matched well.

Key words: fingerprint, Flos lonicera , Radix
Scutellariae, Yinhuang Oral Liquid, high performance liquid chromatography （HPLC）