Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2011, Vol. 29 ›› Issue (12): 1236-1239.DOI: 10.3724/SP.J.1123.2011.01236

• Technical Notes • Previous Articles     Next Articles

Determination of phthalate plasticizers in foods by high performance liquid chromatography with gel permeation chromatographic clean-up

ZHANG Chunyu1, WANG Hui2, ZHANG Xiaohui1, MA Zhongqiang1, DENG Wanmei1, HU Ke1, DING Mingyu2*   

  1. 1. Beijing Labtech Group, Beijing 101312, China; 2. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2011-08-17 Revised:2011-09-27 Online:2011-12-28 Published:2012-01-15
  • Contact: Ming-Yu DING

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Abstract: A method of gel permeation chromatography-high performance liquid chromatography (GPC-HPLC) was established for the simultaneous determination of 5 main phthalate plasticizers in foods (edible oil, instant noodles, fried pastries, Saqima, etc.). The samples were extracted with petroleum ether in an ultrasonator, purified by a GPC column, and analyzed by HPLC. The chromatographic separation was achieved on a Labtech-C18 column (250 mm×4.6 mm, 5 μm) using acetonitrile and water mixture as the mobile phases in a gradient elution mode. The developed method exhibited a linear correlation coefficient of more than 0.997 and the detection limits of 3.25~13.4 μg/L. The spike recoveries were between 70.4% and 113.6% with the relative standard deviations (RSDs, n=3) of 0.3%~5.8% at the spiked level of 50 mg/L. This method is simple, rapid and practical, and can be used for the simultaneous determination of PAEs in grease food samples.

Key words: grease food, high performance liquid chromatography (HPLC), phthalates, plasticizers, gel permeation chromatography (GPC)