Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2012, Vol. 30 ›› Issue (09): 883-888.DOI: 10.3724/SP.J.1123.2012.05023

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Simultaneous extraction and determination of nine chemically synthetic anticoccidial drug multi-residues in chicken meat using high performance liquid chromatography-tandem mass spectrometry

SHI Zuhao1, ZHANG Xiaoyan1,2, BU Shijin2, CHEN Dawei1, PU Junhua1, GAO Yushi1*, TANG Xiujun1   

  1. 1. Poultry Institute, Chinese Academy of Agricultural Sciences/Supervision, Inspection and Testing Center of Poultry Quality, Ministry of Agriculture (Yangzhou), Yangzhou 225125, China; 2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
  • Received:2012-05-14 Revised:2012-07-01 Online:2012-09-28 Published:2012-09-20
  • Contact: 高玉时,研究员,研究方向为禽产品品质与安全. E-mail: gaoys100@sina.com.

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Abstract: A high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the simultaneous extraction and determination of multi-residues of nine chemically synthetic anticoccidial drugs in chicken meat. Toltrazuril-D3 was used as the internal standard. The sample was extracted by 15 mL acetonitrile/dimethyl sulfoxide (4:1, v/v, containing 2% acetic acid), cleaned-up by a QuEChERS (quick, easy, cheap, effective, rugged and safe) clean-up tube with 150 mg octadecylsilyl (ODS), 100 mg Florisil and 100 mg graphitized carbon black (GCB). After the concentration, the extract was analyzed by HPLC-MS/MS. The results showed that good linearity in the range of 20~150 μg/L for each target compound. The recoveries were between 81.5% and 103.6% with the relative standard deviations lower than 15%. The method is simple, rapid, and suitable to detect the chemically synthetic anticoccidial drug residues with high throughput.

Key words: chemically synthetic anticoccidial drugs, chicken meat, multi-residues, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)