Determination of 250 pesticide residues in vegetables using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2015.09026

Abstract

Abstract:

A multiresidue analytical method for the determination of 250 pesticide residues in vegetables was developed by using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The target compounds were extracted with acetonitrile containing 1%(v/v) acetic acid, purified by a mixed sorbent of MgSO₄, primary secondary amine (PSA), graphitized carbon black (GCB) and C18, separated on a Waters ACQUITY^TM UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μ m) and detected by UPLC-MS/MS. Anhydrous magnesium sulfate was used as a dewatering agent. The effects of the amounts of MgSO₄, PSA, GCB and C18 added on the recoveries of 250 pesticides were investigated. The results showed that the purification effect was best when 300 mg MgSO₄, 200 mg PSA, 10 mg GCB and 100 mg C18 in 2 mL of the extract were added. For the 250 pesticide residues, the limits of detection (LODs) of the method were from 0.01 to 50.00 μ g/kg. The recoveries obtained ranged from 60.1% to 120% at three spiked levels in Chinese chives with the relative standard deviations between 3.5% and 19.5% using matrix matched external standard method. The results showed that the method is able to meet requirements of the multiresidue detection of the 250 pesticides in vegetable. The method has the advantages of rapidity, simplicity, high sensitivity and better purification effect. It is suitable for the rapid determination of the common pesticides in vegetables, and it provides a strong guarantee for the risk assessments of the quality and safety of vegetables.

Key words: pesticide multiresidues, QuEChERS, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), vegetables

CLC Number:

O658

ZHANG Aizhi, WANG Quanlin, CAO Lili, LI Yu, SHEN Hao, SHEN Jian, ZHANG Shufen, MAN Zhengyin. Determination of 250 pesticide residues in vegetables using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(2): 158-164.

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