Simultaneous determination of eight vitamin E isomers and vitamin A in foods by normal-phase high performance liquid chromatography

doi:10.3724/SP.J.1123.2016.02011

Abstract

Abstract:

A synchronous detection method was established by normal-phase (NP)-HPLC to separate and determine eight vitamin E isomers and vitamin A in foods. The sample was pretreated and then separated on a Waters ACQUITY^TM UPLC BEH Amide column (150 mm×3.0 mm, 1.7 μm). The mobile phase consisted of hexane (A, 90%) and tert-butyl methyl ether-tetrahydrofuran-methanol (20 : 10 : 1, v/v/v) (B, 10%). The analytes were detected by UV and fluorescence detectors, identified and quantified with external standards. The linear ranges of tocopherols were from 5.0 mg/L to 60.0 mg/L (r²≥0.9999), and those of tocotrienols and vitamin A were from 0.5 mg/L to 6.0 mg/L (r²≥0.9996), while the LODs of vitamin E isomers and vitamin A were from 20 μg/kg to 60 μg/kg. The method is of high precision, stability and repeatability, and the recoveries were from 79.2% to 114.2%. This method is simple, accurate and suitable for the analysis of vitamin E isomers and vitamin A in foods.

Key words: foods, high performance liquid chromatography (HPLC), vitamin A (VA), vitamin E (VE) isomers

CLC Number:

O658

ZHENG Yibin, HUANG Baifen, REN Yiping. Simultaneous determination of eight vitamin E isomers and vitamin A in foods by normal-phase high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2016, 34(7): 692-696.

References

[1] Müller L, Theile K, Böhm V. Mol Nutr Food Res, 2010, 54(5):731
[2] Kamal-Eldin A, Appelqvist L-A. Lipids, 1996, 31(7):671
[3] Pinheiro-Sant'Ana H M, Guinazi M, Oliveira D S, et al. J Chromatogr A, 2011, 1218:8496
[4] Knecht K, Sandfuchs K, Kulling S E, et al. Food Chem, 2015, 169:20
[5] Sun Y L, Li B R, Li G, et al. Chinese Journal of Analytical Chemistry, 2013, 23(3):612 孙亚兰, 李帮锐, 黎冠, 等. 中国卫生检验杂志, 2013, 23(3):612
[6] Aggarwal B B, Sundaram C, Prasad S, et al. Biochem Pharmacol, 2010, 80:1613
[7] Kornsteiner M, Wagner K-H, Elmadfa I. Food Chem, 2006, 98(2):381
[8] Kramer J K G, Blais L, Fouchard R C, et al. Lipids, 1997, 32(3):323
[9] Xie Y F, Yang Y Y, Liu J J, et al. Chinese Journal of Chromatography, 2013, 31(7):679 谢云峰, 杨悠悠, 刘佳佳, 等. 色谱, 2013, 31(7):679
[10] Gentili A, Caretti F. J Chromatogr A, 2011, 1218:684
[11] Cunha S C, Amaral J S, Fernandes J O, et al. J Agric Food Chem, 2006, 54(9):3351
[12] Grebenstein N, Frank J. J Chromatogr A, 2012, 1243:39
[13] Zhang Y H, Qibule H, Jin Y, et al. Chinese Journal of Chromatography, 2015, 33(3):291 张艳海, 其布勒哈斯, 金燕, 等. 色谱, 2015, 33(3):291
[14] Przygoda K, Wejnerowska G. Ind Crop Prod, 2015, 63:41
[15] Zhang Z, Xu C X, Liu J D. Journal of the Chinese Cereals and Oils Association, 2012, 27(10):109 张征, 徐春祥, 刘佳娣, 等. 中国粮油学报, 2012, 27(10):109
[16] Rizzolo A, Polesello S. J Chromatogr A, 1992, 624:103
[17] GB 5413.9-2010
[18] Liu H Y, Yi J W, Zhong Y T, et al. Chinese Journal of Health Laboratory Technology, 2005, 15(9):1047 刘红河, 尹江伟, 仲岳桐, 等. 中国卫生检验杂志, 2005, 15(9):1047
[19] Xie Y F, Wang H, Liu T, et al. Chinese Journal of Analytical Chemistry, 2014, 42(9):1343 谢云峰, 王浩, 刘佟, 等. 分析化学, 2014, 42(9):1343
[20] EN 12822:2000
[21] Kamal-Eldin A, Görgen S, Pettersson J, et al. J Chromatogr A, 2000, 881:217