Simultaneous determination of eight effective constituents in Naoxinqing crude drug and tablets by high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2016.03025

Abstract

Abstract:

A high performance liquid chromatography-tandem mass spectrometry method (HPLC-MS/MS) was developed for the determination of eight constituents in Naoxinqing crude drug and tablets, including protocatechuate, hyperoside, kaempferol-3-O-β-D-glucopyranoside, kaempferol-3-O-β-D-galactopyranoside, myricetin, quercetin, naringenin and kaempferol. Multiple reaction monitoring (MRM) with negative electrospray ionization (ESI^-) in MS/MS was used for quantification. The eight compounds were separated on a Dikma Diamonsil C₁₈ with the mobile phases of acetonitrile and 0.1% (v/v) formic acid aqueous solution with gradient elution. The eight compounds showed good linearity in their concentration ranges with the correlation coefficients (r) better than 0.99. The recoveries were from 99.0% to 102.1%, and the relative standard deviations (RSDs) were 0.9%-3.3% (n=6). The method is accurate, sensitive and effective. It can be used for the quality control of Naoxinqing crude drug and tablets.

Key words: crude drug, effective constituents, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), Naoxinqing, tablets

CLC Number:

O658

ZHANG Mengyu, XU Yang, SONG Guanshan, XIN Xin, PENG Ying. Simultaneous determination of eight effective constituents in Naoxinqing crude drug and tablets by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(8): 773-777.

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