Abstract:

A high performance liquid chromatographic method to separate the respective enantiomers of paroxetine and its intermediate was developed using chiral mobile phase additive. Separation was performed on a Diamond C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was 0.1% phosphate acid-methanol (65∶35, v/v) containing 0.38 g/L carboxymethyl-β-cyclodextrin and the pH was adjusted to 7.2 by triethylamine.The detection wavelength was set at 210 nm and the temperature was 25 ℃. With this method, paroxetine’s trans/cis isomers and their enantiomers as well as intermediate HFP’s trans/cis isomers and their enantiomers were separated simultaneously. The method is simple, rapid with high resolutions.

Key words: carboxymethyl-β-cyclodextrin (CM-β-CD), chiral mobile phase additive, enantiomeric resolution, paroxetine , high performance liquid chromatography (HPLC)