Abstract:

A reliable and sensitive method was introduced for the analysis of streptomycin in royal jelly. The method
was based on a separation by a C8 analytical column with β-naphthoquinone-4-sulfonate post-column derivatization
and fluorescence detection. The clean-up of the extract was done by solid-phase extraction, firstly with an
octadecyl cartridge and secondly with a cation-exchange cartridge. The linear range was 0.02-0.5 mg/L and the
correlation coefficient was 0.9958. The limits of detection (S/N=3) and quantitation (S/N=10) of streptomycin were
0.005 mg/kg and 0.01 mg/kg, respectively. The recoveries ranged from 84.0%to 104.0% with the relative standard
deviations not larger than 7.9%. The method reduced the possibility of the false positive, and it could meet the
need of the current work.

Key words: post-column derivatization, royal jelly
, streptomycin, high performance liquid chromatography (HPLC)