Determination of estradiol in table sugar by auto-solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2016.03011

Abstract

Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established for the determination of estradiol in table sugar. Sugar samples were dissolved in water, and extracted by alkaline acetonitrile, then cleaned-up using an HLB column. The resulting samples were detected under negative multiple reactions monitoring (MRM) mode, and quantified by the internal standard method. As the results showed, the matrix effects were depressed obviously after the purification. The limit of determination and the limit of quantification of the method were 0.1 μg/kg and 0.5 μg/kg, respectively. For all the tested samples, the average spiked recoveries ranged from 83.9% to 95.1%, and the relative standard deviations (RSDs, n=6) were lower than 10%. The method is quick, easy, effective, sensitive and accurate, and can meet the requirements of the determination of estradiol in table sugar.

Key words: auto-solid-phase extraction (auto-SPE), estradiol, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), table sugar

CLC Number:

O658

ZENG Sanmei, XU Dunming, WEI Yiting, ZHONG Liping. Determination of estradiol in table sugar by auto-solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(7): 703-707.

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