Qualitative and quantitative analysis of five microcystins in water by liquid chromatography-diode array detector/ion trap mass spectrometry

doi:10.3724/SP.J.1123.2011.00212

Abstract

Abstract: A method for the determination of five microcystins (MCs) in water was established using liquid chromatography (LC)-diode array detection (DAD)/ion trap mass spectrometry (IT MS). The five MCs in water were enriched and purified by solid phase extraction, and determined by LC-DAD/IT MS. DAD was used for both qualitative and quantitative analysis, and IT MS was used for qualitative analysis only. Under the optimized conditions, the detection limits of 5 MCs in water were 0.1 μg/L. The average recoveries of the three spiked levels (0.2, 0.8 and 5 μg/L) were 52.2%~115.2% with the relative standard deviations (RSDs) of 1.2%~10.0%. This method uses UV absorption spectrum and MS spectrum in the qualitative and quantitative analysis, and can be applied in the determination of various MCs in both surface water and drinking water.

Key words: diode array detection (DAD), ion trap mass spectrometry (IT MS), liquid chromatography (LC), microcystin, water, solid phase extraction

CLC Number:

O658

WANG Chao*, L Yibing, XU Renji, CHEN Ye, YIN Kun, TENG Enjiang. Qualitative and quantitative analysis of five microcystins in water by liquid chromatography-diode array detector/ion trap mass spectrometry[J]. Chinese Journal of Chromatography, 2011, 29(03): 212-216.

References

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