Chinese Journal of Chromatography ›› 2022, Vol. 40 ›› Issue (2): 123-129.DOI: 10.3724/SP.J.1123.2021.04016
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Received:
2021-04-16
Online:
2022-02-08
Published:
2022-01-26
Contact:
ZHANG Jian
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CLC Number:
GUO Minkang, ZHANG Jian. Lipid metabolomic analysis in exosomes of osteonecrosis of the femoral head based on ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2022, 40(2): 123-129.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2021.04016
Character | Age/year | Gender(male/female) | Height/cm | Weight/kg | BMI/(kg/m2) |
---|---|---|---|---|---|
FNF | 72.28±8.27 | 12/18 | 156.47±5.68 | 56.16±8.44 | 22.03±2.51 |
ONFH | 65.57±5.14 | 17/13 | 163.43±5.54 | 61.78±7.94 | 23.62±3.27 |
P | 0.063 | 0.196 | 0.035 | 0.036 | 0.063 |
Table 1 Clinical information of the enrolled samples (n=30)
Character | Age/year | Gender(male/female) | Height/cm | Weight/kg | BMI/(kg/m2) |
---|---|---|---|---|---|
FNF | 72.28±8.27 | 12/18 | 156.47±5.68 | 56.16±8.44 | 22.03±2.51 |
ONFH | 65.57±5.14 | 17/13 | 163.43±5.54 | 61.78±7.94 | 23.62±3.27 |
P | 0.063 | 0.196 | 0.035 | 0.036 | 0.063 |
Fig. 1 Tissue and immunohistochemical imagesof femoral head a. X-ray image of osteonecrosis of femoral head; b. magnetic resonance imaging (MRI) image of osteonecrosis of the femoral head; c. bone tissue specimen of femoral head; d. hematoxylin-eosin (HE) staining of bone tissues.
Fig. 2 Identification of exosomes (EXO) a. exosomes particle size distribution; b. western blotting analysis of markers in EXO; c. transmission electron microscope (TEM) picture of EXO.
Fig. 3 (a) Principal component analysis (PCA) and (b) orthogonal partial least squares discrimination analysis (OPLS-DA) score plots of exosomes samples from FNF and ONFH patients
Metabolite | m/z (Da) | tR/s | VIP | P | HMDB ID | Chemical formula | Average Mr |
---|---|---|---|---|---|---|---|
Phosphatidylserine | 424.279 | 462.799 | 2.170 | 0.014 | HMDB0014291 | C13H24NO10P | 385.304 |
1-(O2-(2-methylamino-2-oxo-ethyl)-O5-hydroxy- | 432.262 | 460.255 | 3.215 | 0.008 | HMDB0013031 | C9H13N5O3 | 239.231 |
phosphinyl-β-d-ribofuranosyl) thymine | |||||||
LysoPE(18∶3(6Z,9Z,12Z)/0∶0) | 440.256 | 461.135 | 1.192 | 0.000 | HMDB0011508 | C23H42NO7P | 475.555 |
Lucyoside K | 597.376 | 336.932 | 1.043 | 0.001 | HMDB0041353 | C36H56O9 | 632.824 |
Physapruin B | 641.289 | 538.855 | 3.067 | 0.016 | HMDB0040671 | C34H50O9 | 602.755 |
Carfentrazone-ethyl | 435.191 | 536.218 | 1.768 | 0.000 | HMDB0041477 | C8H18S | 146.294 |
LysoPC(18∶2(9Z,12Z)) | 520.339 | 563.929 | 1.040 | 0.014 | HMDB0010386 | C26H50NO7P | 519.651 |
1-Palmitoyl-Sn-Glycero-3-Phosphocholine | 496.340 | 589.061 | 3.241 | 0.000 | HMDB0010382 | C24H50NO7P | 495.630 |
LysoPC(20∶4(5Z,8Z,11Z,14Z)) | 544.339 | 605.876 | 1.518 | 0.001 | HMDB0010395 | C28H50NO7P | 543.672 |
1-Oleoyl-Sn-Glycero-3-Phosphocholine | 522.356 | 605.999 | 1.304 | 0.000 | HMDB0002815 | C26H52NO7P | 521.667 |
(±)-Abscisic acid | 265.151 | 359.008 | 1.277 | 0.023 | HMDB0035140 | C15H20O4 | 264.316 |
Methyl methylthio selenide | 142.948 | 72.309 | 1.957 | 0.000 | HMDB0030879 | C2H6SSe | 141.09 |
Pubescenol | 492.321 | 365.979 | 1.648 | 0.000 | HMDB0030085 | C28H42O6 | 474.629 |
Nonacosan-10-one | 423.788 | 372.576 | 3.079 | 0.000 | HMDB0033719 | C29H58O | 422.770 |
(24E)-3α-Acetoxy-15α-hydroxy-23-oxo-7,9(11), | 491.321 | 371.631 | 2.808 | 0.000 | HMDB0035386 | C32H46O6 | 526.704 |
24-lanostatrien-26-oic acid | |||||||
Ganoderic acid Mg | 620.406 | 367.133 | 2.732 | 0.016 | HMDB0035999 | C35H54O8 | 602.798 |
PA (8∶0/12∶0) | 498.310 | 368.099 | 1.056 | 0.005 | HMDB0115483 | C23H45O8P | 480.579 |
Taurolithocholic acid | 484.316 | 366.788 | 1.056 | 0.005 | HMDB0000722 | C26H45NO5S | 483.71 |
Table 2 List of the identified differential metabolites in exosomes
Metabolite | m/z (Da) | tR/s | VIP | P | HMDB ID | Chemical formula | Average Mr |
---|---|---|---|---|---|---|---|
Phosphatidylserine | 424.279 | 462.799 | 2.170 | 0.014 | HMDB0014291 | C13H24NO10P | 385.304 |
1-(O2-(2-methylamino-2-oxo-ethyl)-O5-hydroxy- | 432.262 | 460.255 | 3.215 | 0.008 | HMDB0013031 | C9H13N5O3 | 239.231 |
phosphinyl-β-d-ribofuranosyl) thymine | |||||||
LysoPE(18∶3(6Z,9Z,12Z)/0∶0) | 440.256 | 461.135 | 1.192 | 0.000 | HMDB0011508 | C23H42NO7P | 475.555 |
Lucyoside K | 597.376 | 336.932 | 1.043 | 0.001 | HMDB0041353 | C36H56O9 | 632.824 |
Physapruin B | 641.289 | 538.855 | 3.067 | 0.016 | HMDB0040671 | C34H50O9 | 602.755 |
Carfentrazone-ethyl | 435.191 | 536.218 | 1.768 | 0.000 | HMDB0041477 | C8H18S | 146.294 |
LysoPC(18∶2(9Z,12Z)) | 520.339 | 563.929 | 1.040 | 0.014 | HMDB0010386 | C26H50NO7P | 519.651 |
1-Palmitoyl-Sn-Glycero-3-Phosphocholine | 496.340 | 589.061 | 3.241 | 0.000 | HMDB0010382 | C24H50NO7P | 495.630 |
LysoPC(20∶4(5Z,8Z,11Z,14Z)) | 544.339 | 605.876 | 1.518 | 0.001 | HMDB0010395 | C28H50NO7P | 543.672 |
1-Oleoyl-Sn-Glycero-3-Phosphocholine | 522.356 | 605.999 | 1.304 | 0.000 | HMDB0002815 | C26H52NO7P | 521.667 |
(±)-Abscisic acid | 265.151 | 359.008 | 1.277 | 0.023 | HMDB0035140 | C15H20O4 | 264.316 |
Methyl methylthio selenide | 142.948 | 72.309 | 1.957 | 0.000 | HMDB0030879 | C2H6SSe | 141.09 |
Pubescenol | 492.321 | 365.979 | 1.648 | 0.000 | HMDB0030085 | C28H42O6 | 474.629 |
Nonacosan-10-one | 423.788 | 372.576 | 3.079 | 0.000 | HMDB0033719 | C29H58O | 422.770 |
(24E)-3α-Acetoxy-15α-hydroxy-23-oxo-7,9(11), | 491.321 | 371.631 | 2.808 | 0.000 | HMDB0035386 | C32H46O6 | 526.704 |
24-lanostatrien-26-oic acid | |||||||
Ganoderic acid Mg | 620.406 | 367.133 | 2.732 | 0.016 | HMDB0035999 | C35H54O8 | 602.798 |
PA (8∶0/12∶0) | 498.310 | 368.099 | 1.056 | 0.005 | HMDB0115483 | C23H45O8P | 480.579 |
Taurolithocholic acid | 484.316 | 366.788 | 1.056 | 0.005 | HMDB0000722 | C26H45NO5S | 483.71 |
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